Composite

Part:BBa_K143075

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-10-27)

B. subtilis AmyE locus GFP output promoter and RBS tester


AmyE 3' Integration sequence(BBa_K143002) coupled to GFP protein coding region and the registry double terminator (part BBa_I13401).


The amyE 3' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 5' amyE integration sequence(BBa_K143001) is cloned onto the 5' end of the construct.


The GFP coding region gives a fluorescent output when a promoter and RBS are placed immediately upstream of the coding region while the terminator prevents readthrough.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
//chassis/prokaryote/bsubtilis
Parameters
None